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Agrarian Science and Food Technologies

space SCIENTIFIC JOURNALS OF VINNITSA NATIONAL AGRARIAN UNIVERSITY

Issue №: 1(100)

Published: 2018.04.12
DOI: XX.XXXXX/XXXXXXXXXXXXX


Description:
Аграрна наука та харчові технології. / редкол. В.А.Мазур (гол. ред.) та ін. – Вінниця.: ВЦ ВНАУ, 2018. – Вип. 1(100) – 189 с. Видається за рішенням Вченої ради Вінницького національного аграрного університету (протокол № 11 від « 12 » квітня 2018 року). Дане наукове видання є правонаступником видання Збірника наукових праць ВНАУ, яке було затверджено згідно до Постанови президії ВАК України від 11 вересня 1997 року. Збірник наукових праць внесено в Перелік наукових фахових видань України з сільськогосподарських наук (зоотехнія) (Наказ Міністерства освіти і науки України № 515 від 16 травня 2016 року). У збірнику висвітлено питання підвищення продуктивності виробництва продукції сільського і рибного господарства, технології виробництва і переробки продукції тваринництва, харчових технологій та інженерії, водних біоресурсів і аквакультури. Збірник розрахований на наукових співробітників, викладачів, аспірантів, студентів вузів, фахівців сільського і рибного господарства та харчових виробництв. Прийняті до друку статті обов’язково рецензуються членами редакційної колегії, з відповідного профілю наук або провідними фахівцями інших установ. За точність наведених у статті термінів, прізвищ, даних, цитат, запозичень, статистичних матеріалів відповідальність несуть автори. Свідоцтво про державну реєстрацію друкованого засобу масової інформації КВ № 21523-11423Р від 18.08.2015

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PROVING OF THE MODIFIED PECTIN’S HARMLESSNESS

DOI: XX.XXXXX/XXXXXXXXXXXXX
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Vovkohon A.H., Candidate of Agricultural Science, Associate Professor

e-mail: alinavovk1@ukr.net

Bilotsrkivskiy National Agrarian University

Annotation

The technology of producing traditional sour-milk drinks involves the use of yeasts containing microorganisms. These bioobjects are not always resistant to the inhibitory factors contained in the milk and act from the outside. Reducing the effect of fermentation complicates the technology of making dairy products. To overcome these problems, it is possible to stabilize (immobilize) leaven on organic carriers. For this purpose, natural carriers of protein, lipid and hydrocarbon nature are used. Among carriers of hydrocarbon nature, pectin has a widespread distribution, which possesses sorption and gel-forming properties.

In order to increase the number of reactive groups on the surface of pectin, which would increase its adsorption properties, its modification was carried out using physical and chemical methods. For further use of modified pectin as a nutritional supplement, preclinical studies are required. The indicators of harmlessness of modified pectin remain unstudied.

The purpose of the research is to test the obtained modified pectin in experimental animals (linear mice) as a carrier for the immobilization of cells and enzymes, leaven for sour milk drinks.

The harmlessness of the modified pectin was determined on linear mice. Mice from the control group were intraperitoneally injected with 0.3 ml of physiological saline solution. Animals from the 1st experimental group received 0.3 ml of 5.0% suspension of modified pectin. Mice from the second experimental group were intraperitoneally injected with 0.3 ml of 10.0% suspension of modified pectin.

Observation over experimental mice during a decade showed that deaths of animals in all groups were not noted. It was found that general inhibition was observed during the first 4-7 hours after administration of the modified pectin suspension in mice in the second experimental group. In 80.0% of mice from this experimental group, a disorder of the function of the gastroenteric tract was observed for 24 hours. Over time, the mice recovered active mobility, responded well to external factors (sounds, light and touches), regularly ate food and drank water at will.

Only the absence of appetite was detected within 5-8 hours in mice from the first experimental group. According to pathologic-anatomical investigations of the internal organs of animals, it was found out that the tongue, esophagus, stomach and intestines in mice from experimental groups did not differ from the digestive organs of control group mice. Similarly, no morphological changes in the heart, liver, kidneys, spleen, and lungs in mice of both experimental groups were detected.

No changes in the activity of aminotransferases in the liver of white mice were detected after the administration of high doses of modified pectin.

The protein content of the test animals in the liver was 41.843.0%. Indicators in animals from experimental groups did not have a significant difference in protein content in the liver of mice from the control group. Thus, it has been established that intragastric administration of 5.0% and 10.0% of the modified pectin suspension (0,3 ml/animal) does not affect the protein metabolism in the liver of experimental mice. 

Keywords: modified pectin, experimental animals, total protein, aspartate aminotransferase, alanine aminotransferase, determination of harmlessness, liver of mice

List of references

1. Biotekhnolohiya / V.H. Herasymenko, M.O. Herasymenko, M.I. Tsvilikhovs'kyy ta in.; Za red. V.H. Herasymenka. – K.:INKOS, 2006. – 647 s.

2. Bátori V. Production of Pectin-Cellulose Biofilms: A New Approach for Citrus Waste Recycling /V. Bátori, M. Jabbari, D. Аkesson, P. R. Lennartsson et al // International Journal of Polymer Science. – 2017. – P. 456-465.

3. Srivastava P. Extraction, characterization and evaluation of orange peel waste derived pectin as a pharmaceutical excipient/ P. Srivastava, R. Malviya // J. Natural Products. – 2011. – Vol. 1. – 1. – P. 65-70.

4. Oliveira T. Í. S. Optimization of pectin extraction from banana peels with citric acid by using response surface methodology / T. Í. S. Oliveira, M. F. Rosa, F. L. Cavalcante et al //Food Chemistry. – 2016. – Vol. 198. – P. 113-118.

5. Doklinichni doslidzhennya veterynarnykh likars'kykh zasobiv / [Kotsyumbas I.Ya., Malyk O.H., Patereha I.P. ta in. ]; pid red. I.Ya. Kotsyumbasa. – L'viv: Triada plyus, 2006. – 360 s.

6. Lowry O.H. Protein meashurement with the Folin phenol refgent / O.H. Lowry, N. I. Rosenbrough, A.L. Farr // J. Biol. Chem. – 1951. – Vol. 193. – P. 265-315.

7. Reitman S., A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases / Reitman S., Frankel S..// Amer. J. Clin. Pthol. – 1957. – Vol. 28. – P. 56.

 

 

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About journal

Topics of the journal:

Аnimal feeding and feed technology; current problems of animal breeding, breeding and hygiene; food safety and food processing technology; aquatic bioresources and aquaculture

Key information:
ISSN (print): 2616-728X
DOI: -------

 Certificate of state registration of the print media: КВ № 21523-11423Р 18.08.2015

 Founder of the collection: Vinnytsia National Agrarian University, Georgia Academy of Agricultural Sciences

 Editor-in-Chief: Viktor Mazur, Candidate of Agricultural Sciences Associate Professor, VNAU (Vinnytsia)

 Deputy editors-in-chief: Aleksidze G.M., D.Sc.(Ph.D.), academician of the Academy of agricultural sciences of Georgia; Yaremchuk O.S., D.Sc.(Ph.D.) Professor, University of Vinnitsa (Vinnytsia)

 Secretary in charge: L. Kazmiruk

 Editorial Board full list

Editorial board

Mazur Viktor, Candidate of Science, Associate Professor of VNAU (Editor-in-Chief);

Guram Aleksidze, Ph.D., Academician of the Academy of Agricultural Sciences Georgia (Deputy Editor-in-Chief);

Yaremchuk Oleksandr, Ph.D., Professor, VNAU (Deputy Editor-in-Chief);

Members of the Editorial Board:

Ibatullin Ildus, Ph.D., Professor, Academician, NULES;

Kaletnik Hryhoriy Ph.D. (Economics), Academician of NAAS of Ukraine, VNAU;

Zakharenko Mykola Oleksandrovych, Ph.D., Professor, NULES;

Vashakidze Archil, Ph.D., Academician, National Coordinator for Agricultural Electrification and Automation (Georgia);

Giorgadze Anatoly, Ph.D. (Engineering), Academy of Agricultural Sciences of Georgia;

Grib Josip, Ph.D, Professor of NUHPP;

Dzhaparidze Givi, Ph.D. (Economics), Academician, Vice-President of the Academy of Agricultural Sciences science of Georgia;

Georgiy Yeresko, Ph.D. (Engineering), Professor, Corresponding Member of NAAS of Ukraine, Institute of Food Resources;

Vlasenko Volodymyr, Ph.D.(Engineering), Professor of VTEI;

Kulyk Mykhaylo, Ph.D., Professor, Corresponding Member of NAAS of Ukraine, VNAU;

Kucheriaviy Vitaliy, Ph.D.(Engineering), Professor, VNAU;

Lysenko Alexander, Ph.D., Professor, Research Institute of Experimental Veterinary Science, Academy of Sciences of Belarus (Minsk);

Lyotka Halyna, Ph.D., Associate Professor of VNAU;

Mazurenko Mykola, Ph.D., (Engineering), Professor;

Polishchuk Galina, Ph.D., Associate Professor;

Sychevsky Mykola, Ph.D.(Economics), Professor, Corresponding Member of the National Academy of Sciences of Ukraine, Institute of Food Resources;

Skoromna Oksana, Ph.D., Associate Professor;

Chagelishvili Revaz, Ph.D., Academician, National Forestry Coordinator (Georgia);

Chudak Roman, Ph.D., (Engineering), Professor, VNAU;

Sheiko Ivan, Doctor of Science, Professor, Institute of Animal Science of the Academy of Sciences of Belarus (Zhodino);

Kazmyruk Larisa, Ph.D., Associate Professor of VNAU (secretary in charge).

Edition: Collection

Type of publication for the intended purpose: scientific

Edition status: shared

Year of foundation: 2015

Frequency: 1 to 5 times a year

Volume: 4 to 10 ppm (format A-4)

Edition language: (different languages) Ukrainian, Georgian, English, Russian

 

Scope and category of readers: nationwide, foreign researchers, university professors, graduate students, students and professionals of agriculture and fisheries and food production

Article publishing is a paid service. Cost for printing in professional publications in accordance with the Decree of the Cabinet of Ministers of Ukraine of August 27, 2010 No. 796 "On approval of the list of paid services that can be provided by state educational institutions, other institutions of educational system belonging to state and communal ownership" and the Rector Order of the Higher Education University Mazur V.A. dated March 14, 2018 No. 93 “On setting the cost of printing in professional editions of the University”.

The cost of printing one page in professional editions of the university is 60 UAH.

 

Beneficiary: Vinnytsia National Agrarian University MFO 820172 Current account No. 31256282102055 code 00497236 Bank State Treasury Service of Ukraine, Kyiv. VAT payer code 004972302286. Registration number 100271744. Purpose of payment: for printing an article in the collection: "Agrarian science and food technology".

History of journal:

Agrarian Science and Food Technology  Established in 1994 under the name of the Collection of Scientific Works of VSAU.

According to the Resolution of the Presidium of the Higher Attestation Commission of Ukraine of September 11, 1997, this publication has acquired the status of a professional, in which it is allowed to publish the main results of the dissertation research in the fields of "Agronomy", "Animal engineering" and "Economic Sciences".

In 2001-2014 it was published under the title "Collection of scientific works of Vinnytsia National Agrarian University".

Since 2015 it is:"Agricultural Science and Food Technologies".

 

Listed in the updated list of scientific professional editions of Ukraine in agricultural sciences under the name "Agrarian Science and Food Technologies" (ground: Order of the Ministry of Education and Science of Ukraine of May 16, 2016 No. 515).