logo

Agrarian Science and Food Technologies

space SCIENTIFIC JOURNALS OF VINNITSA NATIONAL AGRARIAN UNIVERSITY

Issue №: 4(107). Т.2

Published: 2019.11.29
DOI: XX.XXXXX/XXXXXXXXXXXXX


Description:
У збірнику висвітлено питання підвищення продуктивності виробництва продукції сільського і рибного господарства, технології виробництва і переробки продукції тваринництва, харчових технологій та інженерії, водних біоресурсів і аквакультури.

Read about journal

PATHOGENICITY STUDY OF TUBERCULOSIS MYCOBACTERIUM ISOLATED FROM TUBERCULIN ON APM-VINTUB MEDIUM

DOI: XX.XXXXX/XXXXXXXXXXXXX
PDF Повернутись

Voitsitskaya O.N., Assistant

 

Vinnytsia National Agrarian University

Annotation

Mycobacterium tuberculosis is a species of pathogenic bacteria first discovered by Robert Koch; it has changed greatly since its discovery. The polymorphism of the pathogens of tuberculosis is the cause of improper use of antibacterial drugs. The researchers have suggested that the mycobacterium tuberculosis polymorphism may be caused by the cyclicity of the microorganism.

Nowadays the most commonly used method of tuberculosis laboratory diagnosis is microbiological examination of the material, i.e. bacterioscopy and seeding on nutrient media. The bacterioscopic method of investigation remains one of the main ones. The advantage of this method is its speed. However, the specificity and sensitivity of bacterioscopy is quite low.

Speaking about methods of research, we should mention that the the most common practice is the sowing of pathological material on such Löwenstein–Jensen medium, Finn-2 medium and others. Bacteriological diagnosis of tuberculosis is quite time-consuming. In addition to the fact that the growth of colonies occurs after a considerable period of time, ranging from 3 weeks to 3 months, the epizootic situation is complicated by the polymorphism of the tuberculosis agent. Known nutrient media began to lose their diagnostic value.

Mycobacteria are very resistant to the environmental factors; they are characterized by a diversity of forms, a large polymorphism and a wide range of variability of biological properties (pleomorphism). The variability of mycobacterium can be observed in the forms of morphological changes (described by I.I. Mechnikov); tinctorial variability, changes caused by colouring (non-Til-Nielsen dye, i.e., loss of acid and alcohol resistance); cultural variability, changes in the shape, size, consistency and color of colonies with growth in artificial nutrient media; biological variability, a change in the degree of virulence in the direction of increase, or decrease it until complete loss.

 

The article presents the results of the study of a new nutrient medium of APM-Vintub and the possibility of cultivation of adaptive forms of the tuberculosis pathogen on it.

Keywords: diagnosis of tuberculosis, mycobacteria, tuberculin, nutrient medium APM-Vintub

List of references

1. Peshkov, M.A. (1954). O tak nazуvaemуkh L-formakh bakteryi [About the so-called L-forms of bacteria]. Microbiology, V. 23, N. 5. S. 607-628 [in Russian].

2. Timakov, V.D., &, Kagan, G.Ya. (1961). Biology of L-forms of bacteria [Byolohyia L-form bakteryi].M.: Medgiz [in Russian].

3. Prozorovsky, S.V. (1970). Problemу patohennosty L-form bakteryi y mykoplazm [Problems of the pathogenicity of L-forms of bacteria and mycoplasmas]. Moscow [in Russian].

4. Ilyina, T.Ya., Zhingreev A.A., Sidorenko O.A. (2008). Osobennosty bakteryovыdelenyia y chuvstvytelnosty mykobakteryi tuberkuleza k khymyopreparatam u bolnыkh s retsydyvamy tuberkuleza lehkykh [Features of bacterial excretion and sensitivity of mycobacterium tuberculosis to chemotherapy in patients with relapse of pulmonary tuberculosis] T.Ya. Ilyina,. Problems of tuberculosis and lung diseases № 5.                              S. 20-22. Moscow [in Russian].

5. Prozorovsky, S.V. (1970). Problemy patohennosty L-form bakteryi y mykoplazm [Problems of the pathogenicity of L-forms of bacteria and mycoplasmas]. Moscow [in Russian].

6. Mattman, L.H., Tunstall, L.H., Mathews, W.W., &, Cordon, D.L. (1960). L-variation in mycobacteria [L-variation in mycobacteria]. Amer. Rev. resp. Dis., v. 82, p. 202-211 [USA].

7. Palіy, A.P. (2013). Epizootolohichnyi monitorynh tuberkulozu velykoi rohatoi khudoby ta naukovo-eksperymentalne obgruntuvannia rozrobky i zastosuvannia zasobiv dezinfektsii [Epizootological monitoring of tuberculosis of great horny thinness and scientific and experimental research and development of knowledge and disinfection]. Kharkiv                        [in Ukrainian].

8. Zavgorodnіy, A.I., Palіy, A.P., &, Stegniy B.T. (2014). Biolohichni vlastyvosti L-form mikobakterii, vydilenykh iz biomaterialu [Biological power of L-forms microbacterium, species of biomaterial]. Veterinary medicine of Ukraine. N. 11. S. 9-12. [in Ukrainian].

9. Vlasenko, I.G. (2009). Detektsiia zbudnyka tuberkulozu v systemi krovi [Detection of an intestine of tuberculosis in the blood system]. Vinnitsya: Edelweiss [in Ukrainian].

10. Lysenko, A.P., Lemish, A.P., et al. (2008). Vыiavlenye adaptyvnыkh form mykobakteryi v tuberkulynakh [Identification of adaptive forms of mycobacteria in tuberculins]. Modern problems of human infectious pathologies. Collection of scientific papers. V. 1. Minsk: «Belprint» p. 227-230. [in Ukrainian].

All journal issues

About journal

Topics of the journal:

Аnimal feeding and feed technology; current problems of animal breeding, breeding and hygiene; food safety and food processing technology; aquatic bioresources and aquaculture

Key information:
ISSN (print): 2616-728X
DOI: -------

 Certificate of state registration of the print media: КВ № 21523-11423Р 18.08.2015

 Founder of the collection: Vinnytsia National Agrarian University, Georgia Academy of Agricultural Sciences

 Editor-in-Chief: Viktor Mazur, Candidate of Agricultural Sciences Associate Professor, VNAU (Vinnytsia)

 Deputy editors-in-chief: Aleksidze G.M., D.Sc.(Ph.D.), academician of the Academy of agricultural sciences of Georgia; Yaremchuk O.S., D.Sc.(Ph.D.) Professor, University of Vinnitsa (Vinnytsia)

 Secretary in charge: L. Kazmiruk

 Editorial Board full list

Editorial board

Mazur Viktor, Candidate of Science, Associate Professor of VNAU (Editor-in-Chief);

Guram Aleksidze, Ph.D., Academician of the Academy of Agricultural Sciences Georgia (Deputy Editor-in-Chief);

Yaremchuk Oleksandr, Ph.D., Professor, VNAU (Deputy Editor-in-Chief);

Members of the Editorial Board:

Ibatullin Ildus, Ph.D., Professor, Academician, NULES;

Kaletnik Hryhoriy Ph.D. (Economics), Academician of NAAS of Ukraine, VNAU;

Zakharenko Mykola Oleksandrovych, Ph.D., Professor, NULES;

Vashakidze Archil, Ph.D., Academician, National Coordinator for Agricultural Electrification and Automation (Georgia);

Giorgadze Anatoly, Ph.D. (Engineering), Academy of Agricultural Sciences of Georgia;

Grib Josip, Ph.D, Professor of NUHPP;

Dzhaparidze Givi, Ph.D. (Economics), Academician, Vice-President of the Academy of Agricultural Sciences science of Georgia;

Georgiy Yeresko, Ph.D. (Engineering), Professor, Corresponding Member of NAAS of Ukraine, Institute of Food Resources;

Vlasenko Volodymyr, Ph.D.(Engineering), Professor of VTEI;

Kulyk Mykhaylo, Ph.D., Professor, Corresponding Member of NAAS of Ukraine, VNAU;

Kucheriaviy Vitaliy, Ph.D.(Engineering), Professor, VNAU;

Lysenko Alexander, Ph.D., Professor, Research Institute of Experimental Veterinary Science, Academy of Sciences of Belarus (Minsk);

Lyotka Halyna, Ph.D., Associate Professor of VNAU;

Mazurenko Mykola, Ph.D., (Engineering), Professor;

Polishchuk Galina, Ph.D., Associate Professor;

Sychevsky Mykola, Ph.D.(Economics), Professor, Corresponding Member of the National Academy of Sciences of Ukraine, Institute of Food Resources;

Skoromna Oksana, Ph.D., Associate Professor;

Chagelishvili Revaz, Ph.D., Academician, National Forestry Coordinator (Georgia);

Chudak Roman, Ph.D., (Engineering), Professor, VNAU;

Sheiko Ivan, Doctor of Science, Professor, Institute of Animal Science of the Academy of Sciences of Belarus (Zhodino);

Kazmyruk Larisa, Ph.D., Associate Professor of VNAU (secretary in charge).

Edition: Collection

Type of publication for the intended purpose: scientific

Edition status: shared

Year of foundation: 2015

Frequency: 1 to 5 times a year

Volume: 4 to 10 ppm (format A-4)

Edition language: (different languages) Ukrainian, Georgian, English, Russian

 

Scope and category of readers: nationwide, foreign researchers, university professors, graduate students, students and professionals of agriculture and fisheries and food production

Article publishing is a paid service. Cost for printing in professional publications in accordance with the Decree of the Cabinet of Ministers of Ukraine of August 27, 2010 No. 796 "On approval of the list of paid services that can be provided by state educational institutions, other institutions of educational system belonging to state and communal ownership" and the Rector Order of the Higher Education University Mazur V.A. dated March 14, 2018 No. 93 “On setting the cost of printing in professional editions of the University”.

The cost of printing one page in professional editions of the university is 60 UAH.

 

Beneficiary: Vinnytsia National Agrarian University MFO 820172 Current account No. 31256282102055 code 00497236 Bank State Treasury Service of Ukraine, Kyiv. VAT payer code 004972302286. Registration number 100271744. Purpose of payment: for printing an article in the collection: "Agrarian science and food technology".

History of journal:

Agrarian Science and Food Technology  Established in 1994 under the name of the Collection of Scientific Works of VSAU.

According to the Resolution of the Presidium of the Higher Attestation Commission of Ukraine of September 11, 1997, this publication has acquired the status of a professional, in which it is allowed to publish the main results of the dissertation research in the fields of "Agronomy", "Animal engineering" and "Economic Sciences".

In 2001-2014 it was published under the title "Collection of scientific works of Vinnytsia National Agrarian University".

Since 2015 it is:"Agricultural Science and Food Technologies".

 

Listed in the updated list of scientific professional editions of Ukraine in agricultural sciences under the name "Agrarian Science and Food Technologies" (ground: Order of the Ministry of Education and Science of Ukraine of May 16, 2016 No. 515).